Regulatory

Part:BBa_K404104:Design

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)

p5-TATAless promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 98
    Illegal BsaI site found at 135


Design Notes

For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested with XbaI and PstI and we ligated it into the iGEM standard plasmid pSB1C3.


Source

pAAV_RC provided from Stratagene

References